Sterilizing AutoclaveInamani ngombwa mu kubungabunga umutekano wa laboratoire no kwemeza ibisubizo nyabyo. Inama zitari sterile zirashobora kumenyekanisha mikorobe, biganisha ku makosa no gutinda mubushakashatsi. Autoclaving ningirakamaro cyane, ikuraho mikorobe nka fungi na bagiteri. Ugereranije nubundi buryo, butanga sterité yuzuye, bigatuma biba ngombwa mubikorwa bya laboratoire byizewe.
Imyiteguro ya Autoclaving Pipette Inama
Ibikoresho bikenewe muri Autoclaving
Kugirango uhagarike neza inama za pipette, ukeneye ibikoresho byiza. Buri gihe ukoreshe inama ya pipette ikozwe muri polypropilene cyangwa copolymers yayo, kuko ibyo bikoresho birashobora kwihanganira autoclaving inshuro nyinshi. Irinde gukoresha inama za polyethylene, kuko zishobora gushonga munsi yubushyuhe bwinshi. Menya neza ko inama zanditseho "Autoclavable" kugirango zemeze ko zikwiye. Byongeye kandi, uzakenera autoclave-umutekano racks cyangwa sterisisation kugirango ufate inama mugihe cyibikorwa. Ibi bikoresho bifasha kugumana ubusugire bwinama no kwemeza ko ikirere gikwirakwira neza.
Kugenzura Inama ya Pipette yo Kwangiza cyangwa Kwanduza
Mbere ya autoclaving, banza ugenzure buri pipette kumutwe, chip, cyangwa ibindi byangiritse bigaragara. Inama zangiritse zirashobora guhungabanya ubukana kandi biganisha kubisubizo bidahwitse. Reba niba hari umwanda usigaye, nk'amazi yumye cyangwa uduce, bishobora kubangamira uburyo bwo kuboneza urubyaro. Hagarika inama zose zerekana ibimenyetso byangiritse cyangwa byanduye kugirango ukomeze ubusugire bwubushakashatsi bwawe.
Gusukura inama zikoreshwa mbere ya Autoclaving
Niba ukoresha inama ya pipette, sukura neza mbere ya autoclaving. Kwoza inama n'amazi yatoboye kugirango ukureho ibisigazwa bya shimi. Kubintu byinangiye byangiza, koresha igisubizo kiboneye kugirango ukureho burundu. Isuku ikwiye ntabwo yongerera imbaraga gusa ahubwo irinda ibisigara kugira ingaruka kumikorere ya autoclave.
Gupakira Pipette Inama muri Autoclave-Yizewe
Shira inama ya pipette mumashanyarazi ya autoclave cyangwa mugihe cyo kuboneza urubyaro. Tegura muburyo butuma umwuka mwiza ugenda neza. Irinde kurenza imitwaro, kuko ibi bishobora kubangamira uburyo bwo kuboneza urubyaro. Niba ukoresha inama zifunze sterile, ntukongere kuzikora, kuko zimaze kuba sterile. Bimaze gutwarwa, menya neza ko ibice byashyizwe neza kugirango wirinde guhindagurika mugihe cyizunguruka.
Imyiteguro ya Autoclaving Pipette Inama
Gushiraho Autoclave
Mbere yo gutangira, menya neza ko autoclave isukuye kandi ikora neza. Reba ikigega cy'amazi hanyuma wuzuze nibiba ngombwa. Kugenzura igipapuro cyumuryango kubimenyetso byose byerekana ko wangiritse cyangwa byangiritse, kuko ibyo bishobora kubangamira inzira. Buri gihe ukurikize amabwiriza yabakozwe kugirango ushireho autoclave neza. Gukoresha autoclave ibungabunzwe neza ituma sterilite yinama yawe kandi ikarinda kwanduzanya.
Guhitamo Inzira Nziza
Guhitamo inzinguzingo ikwiye ningirakamaro muburyo bwo kuboneza urubyaro. Inzinguzingo zisanzwe zirimo:
- Imbaraga rukuruzi: Yishingikiriza kumyuka isanzwe kandi nibyiza kumpanuro ya pipette. Shyira kuri 252 ° F muminota 20 kumurongo umwe wumuvuduko ugereranije.
- Vacuum (prevac) cycle: Koresha icyuho kugirango ukureho umwuka mbere yo kwinjiza umwuka, urebe neza ko winjira neza.
- Inzira y'amazi: Yashizweho kubintu byuzuye amazi ariko ntibisanzwe bikoreshwa muburyo bwa pipette.
Guhitamo inama ya pipette ishobora kwihanganira ibi bintu ni ngombwa kugirango ubungabunge ubusugire bwabo.
Gupakira Autoclave neza
Mugihe urimo gupakira autoclave, ambara ibikoresho byo kurinda umuntu (PPE) nka gants, ibirahure byumutekano, hamwe na kote ya laboratoire. Tegura ibice bifite umwanya uhagije hagati yabyo kugirango wemerere kuzenguruka. Irinde gupakira cyane autoclave, kuko ibyo bishobora kubangamira ingumba. Menya neza ko ibipfundikizo bya tray tray bifunguye gato kugirango umwuka winjire. Ntuzigere upfunyika ibintu muri fayili, kuko ifata ubuhehere kandi ikarinda guhagarika neza.
Gukoresha Autoclave no gukurikirana inzira
Tangira autoclave kandi ukurikirane hafi. Reba ubushyuhe, umuvuduko, nigihe cyigihe kugirango urebe ko byujuje ibisabwa. Koresha ibipimo by'imiti byimbere, nkubwoko bwa 4 cyangwa ubwoko bwa 5, kugirango wemeze ko imiti igabanya ubukana yinjiye mubipfunyika. Gukurikirana imashini, nko kureba ibipimo, bifasha kugenzura ko autoclave ikora neza. Andika inzira yo gukurikiranwa no kwemeza ubuziranenge.
Gukonjesha no gupakurura Autoclave
Umuzenguruko umaze kurangira, emerera autoclave gukonja mbere yo kuyifungura. Reba igipimo cyumuvuduko kugirango urebe ko isoma 0 PSI. Hagarara inyuma yumuryango hanyuma ukingure buhoro kugirango urekure amavuta asigaye neza. Reka inama ya pipette ikonje muburyo busanzwe muri autoclave kugirango ikomeze sterile. Kugira ngo byume vuba, ohereza ibisate muri kabine yumye yashyizwe kuri 55 ° C. Gukonjesha neza no gupakurura birinda kwangirika kumpanuro nziza kandi ubungabunge imikorere yazo.
Nyuma ya Autoclaving Pipette Inama Ikoreshwa nububiko
Kuraho Inama za Pipette Zitunganijwe neza
Gukoresha neza inama ya pipette neza ningirakamaro kugirango ukomeze kutabyara. Buri gihe ujye wambara uturindantoki kugirango wirinde kwanduza uruhu. Koresha gusa ibikoreshwa byanditseho "sterile" kugirango ugabanye ingaruka. Mbere yo gukoresha inama, sukura pipeti hamwe na nyirayo hamwe na 70% Ethanol. Iyi ntambwe iremeza ko nta bihumanya bibangamira sterile yinama. Mugihe ukuyeho inama muri autoclave, irinde kubashyira ahagaragara umwuka mugihe kirekire. Iyimure mu buryo butaziguye mu kintu gisukuye, gifunze cyangwa ibikoresho byabo byumwimerere kugirango ubungabunge ubusugire bwabo.
Kugenzura Inama Zibyangiritse Nyuma ya Sterilisation
Nyuma ya autoclaving, genzura inama ya pipette kubimenyetso byose byangiritse. Reba kurigata, gucamo, cyangwa guhindura ibara, kuko ibyo bibazo bishobora kugira ingaruka kubikorwa byabo. Inama zangiritse zirashobora guhungabanya ukuri kwubushakashatsi bwawe cyangwa kumenyekanisha ibyanduye. Hagarika inama zose zerekana inenge zigaragara. Iyi ntambwe yubugenzuzi yemeza ko gusa inama nziza-nziza, sterile ikoreshwa mubikorwa byawe.
Kubika Inama ya Pipette kugirango Ukomeze Sterility
Kubika neza nibyingenzi kugirango inama za pipette zibe sterile nyuma ya autoclaving. Bika inama mubipfunyika byumwimerere bifunze cyangwa ibikoresho byumuyaga kugirango wirinde kwanduza. Irinde gupfunyika udusanduku twa fayili, kuko ibi bishobora gufata imvura kandi bigatera imikurire ya mikorobe. Shira ibikoresho byabitswe ahantu hakonje, humye kure yizuba ryinshi nubushuhe. Buri gihe usukure agasanduku k'ububiko kugirango ukomeze gukora neza. Iyi myitozo ifasha kwemeza sterile yinama zawe kugeza zikoreshwa ubutaha.
Kuranga no Gutegura Inama Zitandukanijwe
Kwandika no gutunganya inama zawe za sterile zitezimbere kunoza imikorere no kugabanya amakosa. Koresha ibirango bisobanutse kugirango werekane itariki yo guhagarika ubwoko n'ubwoko bw'inama zabitswe. Tegura inama kubunini cyangwa gusaba kugirango byoroshye kuboneka mugihe cyubushakashatsi. Komeza ahantu ho kubika neza kugirango wirinde kwandura impanuka. Ishirahamwe ryiza ntirizigama umwanya gusa ahubwo riremeza ko burigihe ufite inama zidasanzwe ziteguye gukoreshwa.
Amakosa Rusange Iyo Autoclaving Pipette Inama
Kurenza kuri Autoclave
Kurenza urugero autoclave ibangamira uburyo bwo kuboneza urubyaro. Iyo upakiye inama nyinshi za pipette mucyumba, umwuka ntushobora kuzenguruka neza. Ibi bivamo kutabyara kimwe, hasigara inama zimwe na zimwe. Buri gihe utegure inama muri autoclave-umutekano rack hamwe n'umwanya uhagije hagati yabo. Irinde gutondeka neza. Umwanya ukwiye uremeza ko umwuka ugera kuri buri nama, ukagumana ubudakemwa n'ubunyangamugayo.
Gukoresha Igenamiterere rya Autoclave
Igenamiterere ritari ryo rishobora kwangiza inama za pipette cyangwa kunanirwa kuzifata. Kurugero, inama za pipette zigomba kuba autoclave rimwe gusa kuri 121 ° C muminota 10, hanyuma hagakurikiraho kumisha kuri 110 ° C muminota 5. Gukoresha ubushyuhe buri hejuru cyangwa inzinguzingo ndende birashobora gutuma inama zicika cyangwa bigatera gushungura. Ingaruka zijyanye nigenamiterere ridakwiye zirimo:
| Ingaruka z'umutekano | Ibisobanuro |
|---|---|
| Ubushyuhe burashya | Kuva mubikoresho bishyushye hamwe na autoclave chambre n'inzugi |
| Amashyiga arashya | Kuva kumasiga asigaye yarekuwe nyuma yizunguruka |
| Amazi ashyushye | Kuva kumazi yatetse cyangwa kumeneka imbere muri autoclave |
| Gukomeretsa ukuboko n'ukuboko | Iyo ufunze umuryango wa autoclave |
| Gukomeretsa umubiri | Niba hari iturika kubera igitutu kidakwiye cyangwa gupakira |
Buri gihe ukurikize amabwiriza yabakozwe kugirango uhitemo uruziga rukwiye rwa autoclave pipette.
Gusimbuka Intambwe Yambere yo Gusukura
Gusimbuka mbere yo gukora isuku biganisha kubibazo byanduye. Imiti isigaye cyangwa ibikoresho byibinyabuzima kumpanuro yakoreshejwe birashobora kubangamira sterisizione. Ibi birashobora kuvamo:
- Pipette-to-sample yanduye, aho umuyoboro winjiza ibyanduye muri sample.
- Icyitegererezo-kuri-pipette kwanduza, aho icyitegererezo cyanduza umubiri wa pipette.
- Icyitegererezo-kuri-icyitegererezo cyanduye, aho ibisigazwa byimurwa hagati yintangarugero.
Sukura neza inama hamwe namazi yatoboye cyangwa umuti woguhumanya mbere yo kwikora. Iyi ntambwe ningirakamaro mukurinda kwanduzanya no kwemeza ibisubizo byizewe.
Gukemura nabi nyuma ya Sterilisation
Gukemura inama zidakwiye birashobora gukuraho inzira yo kuboneza urubyaro. Buri gihe ujye wambara uturindantoki mugihe ukuyemo inama muri autoclave. Irinde gukora ku nama mu buryo butaziguye cyangwa kuyishyira ahagaragara kugirango ufungure igihe kirekire. Iyimure ako kanya kubikoresho bifunze cyangwa uduce twagenewe gukoreshwa na pipette. Iyi myitozo ifasha kugumana sterile yinama zawe.
Kubika Inama Mubitari Sterile
Kubika inama mubihe bitari sterile bibatera kwanduza. Koresha ibikoresho byumuyaga cyangwa udusanduku twafunze kugirango urinde inama zidasanzwe. Irinde gupfunyika inama muri file, kuko ifata ubuhehere kandi igatera mikorobe. Bika inama ahantu hakonje, humye kugirango ubungabunge sterile hamwe nubushakashatsi bwimiti ya pipette. Ububiko bukwiye butanga ubusugire bwinama zawe zo gukoresha ejo hazaza.
Inama: Buri gihe ugenzure inama zangiritse cyangwa kurwana nyuma ya autoclaving. Inama zangiritse zirashobora guhungabanya ubushakashatsi bwawe kandi biganisha kubisubizo bidahwitse.
Guhindura inama ya pipette ningirakamaro mukubungabunga umutekano wa laboratoire no kwemeza ibisubizo nyabyo. Kuringaniza neza birinda kwanduza, bikomeza ubusugire bwibigeragezo byawe, kandi bigashyigikira ibisubizo byizewe.
Mu ncamake, kurikiza izi ntambwe zo kuboneza urubyaro:
- Witegure kugenzura no gusukura inama za pipette.
- Autoclave ukoresheje igenamiterere ryiza kandi urebe neza ko ikirere gikwirakwira.
- Nyuma yo kuboneza urubyaro, koresha inama witonze kandi ubibike mu bikoresho bifunze kugirango ukomeze kutabyara.
Ibyingenzi byingenzi kumutekano wa laboratoire harimo:
- Koresha autoclave kugirango ukureho mikorobe.
- Ubike inama mubipfunyika byumwimerere cyangwa ibikoresho byumuyaga.
- Kugenzura inama zangiritse mbere yo kuyikoresha kandi wirinde kuyishyira ahagaragara.
Ukurikije iyi myitozo, uremeza kubika no gukoresha inama za sterile sterile, igabanya ingaruka zanduye kandi ikongerera ukuri kugeragezwa.
Igihe cyo kohereza: Gashyantare-19-2025