Pipette yog ib qho ntawm cov cuab yeej siv ntau tshaj plaws siv hauv cov chaw kuaj mob lom neeg, tshuaj lom neeg, thiab tshuaj ntsuam xyuas qhov chaw uas cov kua yuav tsum tau ntsuas kom meej thiab hloov pauv thaum ua cov tshuaj dilutions, kuaj lossis kuaj ntshav.Lawv muaj xws li:
① ib-channel lossis ntau-channel
② tsau lossis adjustable ntim
③ phau ntawv los yog hluav taws xob
Dab tsi yog Single-Channel Pipettes?
Ib leeg-channel pipette tso cai rau cov neeg siv hloov pauv ib qho aliquot ib zaug.Cov no zoo li siv rau hauv cov chaw kuaj mob uas muaj cov qauv qis, uas feem ntau tuaj yeem yog cov koom nrog hauv kev tshawb fawb thiab kev loj hlob.
Ib leeg-channel pipette muaj ib lub taub hau rau aspirate los yog dispense heev npaum li cas ntawm cov kua los ntawm ib tug pov tseg.lus qhia.Lawv tuaj yeem siv rau ntau daim ntawv thov hauv cov chaw kuaj mob uas tsuas muaj me me xwb.Qhov no feem ntau yog cov chaw soj nstuam uas ua cov kev tshawb fawb txog kev tshuaj ntsuam chemistry, kab lis kev cai ntawm tes, noob caj noob ces lossis kev tiv thaiv kab mob.
Multi-Channel Pipettes yog dab tsi?
Multi-channel pipettes ua haujlwm tib yam li ib leeg-channel pipettes, tab sis lawv siv ntau yamtswv yimrau ntsuas thiab faib cov kua sib npaug ntawm ib zaug.Kev teeb tsa feem ntau yog 8 lossis 12 channel tab sis 4, 6, 16 thiab 48 channel teeb kuj muaj.96 channel benchtop versions kuj tuaj yeem yuav.
Siv cov kav dej ntau ntau, nws yooj yim los sau 96-, 384-, lossis 1,536-zoo.microtiter phaj, uas tej zaum yuav muaj cov qauv rau kev siv xws li DNA amplification, ELISA (kev kuaj mob), kev tshawb fawb kinetic thiab kev tshuaj ntsuam molecular.
Ib-Channel vs. Multi-Channel Pipettes
Kev ua tau zoo
Ib leeg-channel pipette yog qhov zoo tagnrho thaum ua haujlwm sim.Qhov no yog vim hais tias nws tsuas yog siv cov leeg ib leeg, lossis ib qho kev sib tw sib tw los ua hauv cov ntshav.
Txawm li cas los xij, qhov no sai sai dhau los ua ib qho cuab yeej tsis muaj txiaj ntsig thaum muaj kev nce ntxiv.Thaum muaj ntau yam qauv / reagents los hloov, lossis kev soj ntsuam loj dua tau khiav hauv96 zoo microtitre daim hlau, muaj ntau txoj hauv kev zoo dua los hloov cov kua dej tom qab siv ib txoj kab pipette.Los ntawm kev siv ntau lub channel pipette xwb, tus naj npawb ntawm cov kauj ruam pipette tau txo qis heev.
Cov lus hauv qab no qhia txog tus naj npawb ntawm cov kauj ruam uas xav tau rau kev teeb tsa ib leeg, 8 thiab 12 channel.
Tus naj npawb ntawm cov kauj ruam uas yuav tsum tau ua (6 reagents x96 Well Microtitre Phaj)
Ib-channel Pipette: 576
8- Channel Pipette: 72
12-Channel Pipette: 48
Qhov Volume ntawm Pipetting
Ib qho tseem ceeb sib txawv ntawm ib leeg thiab ntau-channel pipettes yog qhov ntim ntawm qhov dej uas tuaj yeem hloov mus rau ib zaug.Txawm hais tias nws nyob ntawm tus qauv siv, feem ntau koj tsis tuaj yeem hloov pauv ntau npaum li ib lub taub hau ntawm ntau lub raj xa dej.
Lub ntim ib leeg-channel pipette tuaj yeem hloov pauv tau ntawm 0.1ul thiab 10,000ul, qhov ntau ntawm cov kav dej ntau ntawm 0.2 thiab 1200ul.
Qauv Loading
Keeb kwm, cov kav dej ntau ntau tau ua tsis tau zoo thiab nyuaj rau siv.Qhov no tau ua rau cov qauv thauj khoom tsis sib xws, nrog rau cov teeb meem thauj khoomtswv yim.Muaj cov qauv tshiab muaj tam sim no txawm li cas los xij, uas yog cov neeg siv ntau dua thiab mus qee txoj hauv kev los daws cov teeb meem no.Nws kuj tseem tsim nyog sau cia tias txawm hais tias kev thauj khoom ua kua yuav ua rau me ntsis tsis raug nrog ntau lub channel pipette, lawv yuav muaj feem ntau yog qhov tseeb tag nrho tshaj li ib-channel vim qhov tsis raug uas tshwm sim los ntawm cov neeg siv kev ua yuam kev los ntawm kev qaug zog ( saib kab lus tom ntej).
Txo tib neeg yuam kev
Qhov tshwm sim ntawm tib neeg kev ua yuam kev yog txo qis vim tias tus naj npawb ntawm cov kauj ruam pipet txo qis.Kev hloov pauv los ntawm kev qaug zog thiab kev ntxhov siab raug tshem tawm, ua rau cov ntaub ntawv thiab cov txiaj ntsig uas txhim khu kev qha thiab rov tsim dua tshiab.
Calibration
Txhawm rau kom ntseeg tau qhov tseeb thiab qhov tseeb ntawm cov cuab yeej siv ua kua, yuav tsum tau calibration tsis tu ncua.Tus qauv ISO8655 hais tias txhua channel yuav tsum tau kuaj thiab tshaj tawm.Qhov ntau channels lub pipette muaj, nws yuav siv sij hawm ntev los calibrate uas yuav siv sij hawm.
Raws li pipettecalibration.net tus qauv 2.2 calibration ntawm 12-channel pipette yuav tsum muaj 48 pipetting cycles thiab gravimetric hnyav (2 ntim x 2 repetitions x 12 channels).Nyob ntawm qhov ceev ntawm tus neeg teb xov tooj, qhov no tuaj yeem siv sijhawm ntau dua 1.5 teev ib pipette.Laboratories nyob rau hauv lub tebchaws United Kingdom yuav tsum tau UKAS calibration yuav tsum tau ua ib tug tag nrho ntawm 360 gravimetric hnyav (3 ntim x 10 repetitions x 12 raws).Kev ua tus lej ntawm cov kev xeem no ua tsis tau raws li qhov ua tsis tau thiab yuav ua rau ntau dua lub sijhawm txuag tau los ntawm kev siv ntau lub raj xa dej hauv qee qhov chaw sim.
Txawm li cas los xij, kom kov yeej cov teeb meem no pipette calibration cov kev pab cuam muaj los ntawm ntau lub tuam txhab.Piv txwv ntawm cov no yog Gilson Labs, ThermoFisher thiab Pipette Lab.
Kho
Nws tsis yog ib yam dab tsi uas ntau tus xav txog thaum yuav lub pipette tshiab, tab sis lub manifold ntawm ib co multi-channel pipettes tsis kho.Qhov no txhais tau tias yog 1 channel puas lawm, tag nrho cov manifold yuav tsum tau hloov.Txawm li cas los xij, qee cov tuam txhab muag kev hloov pauv rau ib tus neeg raws, yog li nco ntsoov xyuas kev kho kom zoo nrog cov chaw tsim khoom thaum yuav ib lub raj xa dej ntau.
Summary – Ib leeg vs Multi-Channel Pipettes
Multi-channel pipette yog ib qho cuab yeej tseem ceeb rau txhua qhov chaw kuaj mob uas muaj dab tsi ntau tshaj li qhov me me ntawm cov qauv.Nyob rau hauv yuav luag txhua qhov xwm txheej qhov siab tshaj plaws ntawm cov kua yuav tsum tau hloov mus rau hauv lub peev xwm ntawm txhua tuslus qhianyob rau hauv ib tug multi-channel pipette, thiab muaj tsawg heev drawbacks txuam nrog qhov no.Ib qho me me ntawm qhov nyuaj ntawm kev siv ntau lub kav dej pipette yog qhov ua tau zoo heev los ntawm kev txo qis hauv kev ua haujlwm, ua haujlwm los ntawm kev txo qis qis ntawm cov kauj ruam.Tag nrho cov no txhais tau tias txhim kho cov neeg siv kev nplij siab, thiab txo cov neeg siv yuam kev.
Post lub sij hawm: Dec-16-2022