Automated Liquid tuavhais txog kev siv cov tshuab automated es tsis txhob siv tes ua haujlwm los hloov cov kua nruab nrab ntawm qhov chaw. Hauv cov chaw tshawb fawb lom neeg, cov txheej txheem ua kua hloov pauv ntau los ntawm0.5 μL rau 1 mL, txawm hais tias kev hloov pauv nanoliter yog xav tau hauv qee qhov kev siv. Automated kua tuav tshuab sib txawv ntawm qhov loj, qhov nyuaj, kev ua haujlwm, thiab tus nqi.
Los ntawm phau ntawv mus rau Automated Liquid Handling
Cov cuab yeej yooj yim tshaj plaws yog covphau ntawv pipette-ib lub cuab yeej siv tes ua yuav tsum muaj kev cuam tshuam cov neeg siv rov qab rau txhua kauj ruam (kev cia siab thiab xa tawm). Kev siv sijhawm ntev tuaj yeem ua rau muaj kev raug mob rov qab zoo licarpal qhov syndrome.
Electronic pipettessawv cev rau cov kauj ruam tom ntej evolutionary. Ob phau ntawv thiab hluav taws xob pipettes muaj peev xwm kho tau / tsau ntim thiab 1-16 raws. Txawm hais tias ntau lub channel hluav taws xob pipettes nce kev sib piv piv rau phau ntawv ib leeg-channel pipettes, lawv tseem txwv los ntawm tib neeg cov tswv yim.Automated dispenserkov yeej qhov no los ntawm ib txhij faib cov kua rau hauv txhua qhov dej ntawm lub microplate (piv txwv li, 96- lossis 384-zoo daim hlau).
Cov kev ntsuam xyuas niaj hnub no feem ntau xav tau ntau kauj ruam "kev ua haujlwm."Automated kua tuav workstationsintegrate modules (xws li, shakers, heaters) thiab software los ua cov txheej txheem nyuaj.
- Nkag-theem systemsyog compact nrog cov neeg siv-phooj ywg software tab sis txwv yooj.
- Advanced systemstxhawb kev hloov kho modular, nthuav dav kev ua haujlwm, thiab kev koom ua ke nrog lwm cov khoom siv hauv chav kuaj.
Cov yam tseem ceeb rau kev xaiv cov cuab yeej ua kua muaj xws li:
(i) Kev Tshaj Tawm, (ii) Workflow complexity, (iii) Budget, (iv) Lab space, (v) Sterility/cross-contamination control, (vi) Traceability, (vii) Precision.
Precision hauv Automated Liquid Handling
Precision nyob ntawm cov khoom ua kua, cov txheej txheem pipetting, thiab (rau phau ntawv siv tshuab) cov neeg siv kev txawj. Cov khoom ua kua - cuam tshuam los ntawm qhov kub, siab, thiab av noo - suav nrog:
- viscosity(flow cwj pwm)
- Qhov ntom(mass / unit volume)
- Adhesion/cohesion(kev nplaum)
- Ntog nro
- Vapor siab
Cov txheej txheem Advanced kho cov kev txwv kom suav nrog cov khoom no:
(i) Aspiration / dispensing ceev,
(ii) Air gap (blowout/air displacement),
(iii) Pre-aspiration nyob lub sij hawm,
(iv) Ntsis tshem tawm ceev.
Major Pipetting Technologies
Classified los ntawm cov kua propulsion mechanisms:
- Kev xaav cua
- Kev Ua Kua Dej
- Qhov Zoo Tshaj Plaws
- Acoustic Technology
Evolution ncua sij hawm
Manual Pipette (Sib-Channel) → Phau Ntawv Pipette (Multi-Channel) → Electronic Pipette → Automated Dispenser → Nkag-Level Workstation → Modular Automated Workstation
| Pipetting Technology | Ntsiab nta | Cov ntawv thov tseem ceeb |
| Kev xaav cua | Cua cushion cais lub piston txav ntawm tus qauv | Zoo ruaj khov rau ntim hauv 0.5-1,000 μl |
| Kev Ua Kua Dej | Cua cushion cais cov kua dej los ntawm cov qauv | Feem ntau yog siv nrog cov lus qhia ruaj ruaj stainless hlau washable; zoo tagnrho rau cov kauj ruam uas yuav tsum tau pierced raj |
| Qhov Zoo Tshaj Plaws | Kev sib cuag ncaj qha ntawm kev txav piston thiab cov qauv | Txaus siab rau high-viscosity thiab volatile qauv |
| Acoustic Technology | Kev hloov pauv kua tsis sib cuag nrog lub zog acoustic (suab yoj) | Ultra-tsawg ntim (mus rau nanoliter ntau) |
Post lub sij hawm: May-12-2025