Gudanar da Liquid Mai sarrafa kansayana nufin amfani da tsarin sarrafa kansa maimakon aikin hannu don canja wurin ruwa tsakanin wurare. A cikin dakunan binciken nazarin halittu, daidaitattun adadin canja wurin ruwa daga0.5 zuwa 1 ml, kodayake ana buƙatar canja wurin matakin nanoliter a wasu aikace-aikacen. Tsarin sarrafa ruwa mai sarrafa kansa ya bambanta da girma, rikitarwa, aiki, da farashi.
Daga Manual zuwa Gudanar da Liquid Mai sarrafa kansa
Mafi mahimmanci kayan aiki shinepipette na hannu- na'urar hannu da ke buƙatar maimaituwar sa hannun mai amfani ga kowane mataki (buri da rarrabawa). Yin amfani da dogon lokaci na iya haifar da raunin raunin da ya farucarpal tunnel ciwo.
Lantarki pipetteswakiltar mataki na gaba na juyin halitta. Dukansu pipettes na hannu da lantarki na iya samun daidaitacce / ƙayyadaddun kundin da tashoshi 1-16. Yayin da bututun lantarki na tashoshi da yawa suna haɓaka kayan aiki idan aka kwatanta da pipettes na tashoshi ɗaya na hannu, sun kasance iyakance ta hanyar shigar ɗan adam.Masu rarrabawa ta atomatikshawo kan wannan ta hanyar rarraba ruwa lokaci guda zuwa duk rijiyoyin microplate (misali, faranti 96- ko 384-rijiya).
Gwajin dakin gwaje-gwaje na zamani sau da yawa yana buƙatar “gudun aiki” matakai da yawa.Wuraren sarrafa ruwa mai sarrafa kansahaɗa kayayyaki (misali, masu girgiza, masu dumama) da software don aiwatar da ƙa'idodi masu rikitarwa.
- Tsarin shigarwa-matakinsuna m tare da software mai sauƙin amfani amma iyakancewar sassauci.
- Na gaba tsaringoyi bayan haɓakawa na zamani, faɗaɗa ayyukan aiki, da haɗin kai tare da sauran kayan aikin lab.
Mahimman abubuwan da za a zaɓa fasahar sarrafa ruwa sun haɗa da:
(i) Kayan aiki, (ii) Matsalolin aiki, (iii) Kasafin kuɗi, (iv) Filin Lab, (v) Tsaftacewa / sarrafa gurɓatawar giciye, (vi) Ganowa, (vii) Daidaitawa.
Madaidaici a Gudanar da Liquid Na atomatik
Madaidaici ya dogara da kaddarorin ruwa, dabarar bututu, da (na tsarin hannu) ƙwarewar mai amfani. Kayayyakin ruwa—wanda zafin jiki, matsa lamba, da zafi ya shafa—sun haɗa da:
- Dankowar jiki(halayyar kwarara)
- Yawan yawa(girman taro/raka'a)
- Adhesion/haɗin kai(danko)
- Tashin hankali
- Turi matsa lamba
Na'urori masu tasowa suna daidaita sigogi don lissafin waɗannan kaddarorin:
(i) Gudun buri/bayarwa,
(ii) Gaps na iska (bushewar iska),
(iii) Tsawon lokacin buri,
(iv) Tukwici gudun janyewa.
Manyan Pipetting Technologies
Rarraba ta hanyoyin motsa ruwa:
- Matsar da iska
- Matsar Ruwa
- Matsuwa Mai Kyau
- Fasahar Acoustic
Jadawalin Juyin Halitta
Pipette na Manual (Tashoshi Guda) → Pipette na Manual (Tashoshi da yawa) → Pipette Lantarki → Mai Rarraba Mai sarrafa kansa
| Fasahar Bututu | Mabuɗin Siffofin | Aikace-aikace na farko |
| Matsar da iska | Matashin iska yana raba piston mai motsi daga samfurin | Matsakaicin kwanciyar hankali don juzu'i tsakanin 0.5-1,000 μl |
| Matsar Ruwa | Matashin iska yana raba ruwan tsarin daga samfurin | Yawanci ana amfani da shi tare da ƙayyadaddun tukwici masu wanke bakin karfe; manufa don matakan da ke buƙatar bututun da aka soke |
| Matsuwa Mai Kyau | Sadarwa kai tsaye tsakanin fistan motsi da samfurin | An fi so don babban danko da samfuran maras tabbas |
| Fasahar Acoustic | Canja wurin ruwa mara lamba ta amfani da makamashin sauti (raƙuman sauti) | Ƙananan ƙananan juzu'i (har zuwa kewayon nanoliter) |
Lokacin aikawa: Mayu-12-2025